Informatics Publishing Limited

P. M. Patil ¹, P. D. Chaudhari ¹, Megha Sahu ¹, N. J. Duragkar ²

Affiliations
1 Modern College of Pharmacy, Sector 21, Yamunanagar, Nigdi, Pune-44 (M.S), IN
2 Sharad Pawar College of Pharmacy, Nagpur, IN

Abstract

Gene therapy can be broadly defined as the transfer of genetic material to cure a disease or at least to improve the clinical status of a patient. One of the basic concepts of gene therapy is to transform viruses into genetic shuttles, which will deliver the gene of interest into the target cells. Based on the nature of the viral genome, these gene therapy vectors can be divided into RNA and DNA viral vectors. The majority of RNA virus-based vectors have been derived from simple retroviruses like murine leukemia virus. A major shortcoming of these vectors is that they are not able to transducer nondividing cells. This problem may be overcome by the use of novel retroviral vectors derived from lentiviruses, such as human immunodeficiency virus (HIV). The most commonly used DNA virus vectors are based on adenoviruses and adeno-associated viruses. An example of gene-knockout mediated gene therapy is the knockout of the human CCR5 gene in T-cells in order to control HIV infection. Although the available vector systems are able to deliver genes in vivo into cells, the ideal delivery vehicle has not been found. Thus, the present viral vectors should be used only with great caution in human beings and further progress in vector development is necessary.

Keywords

Lentiviruses, Transducer, RNA Vector, Adeno-Associated Viruses.

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A. V. Jaydeokar ¹, D. D. Bandawane ¹, S. S. Nipate ¹, P. D. Chaudhari ¹

Affiliations
1 Progressive Education Society's Modern College of Pharmacy, Sector No. 21, Yamunanagar, Nigdi, Pune-44, Maharashtra, IN

Abstract

Free radicals cause oxidative damage to lipids, proteins and DNA, eventually leading to many chronic diseases such as cancer, diabetes, aging, and other degenerative diseases in humans. Reactive oxygen species (ROS) such as superoxide anions (O₂^-), hydroxyl radical (OH^-) and nitric oxide (NO) inactivate enzymes and damage important cellular components causing injury through covalent binding and lipid peroxidation. Antioxidants offer resistance against the oxidative stress by scavenging the free radicals, inhibiting the lipid peroxidation and by other mechanisms and thus prevent disease. The evaluation of the antioxidant properties of specific chemical scavengers is of particular value for their potential use in preventing or limiting the damage induced by free radicals. Various kinds of antioxidants particularly from natural sources such as enzymes, tocopherol, carotenoids, ascorbic acid, polyphenols etc. inhibit the cellular damage mainly through free radical scavenging property. Therefore, antioxidant-based drug formulations are used for the prevention and treatment of complex diseases involving free radicals like atherosclerosis, stroke, diabetes, Alzheimer's disease and cancer. This study reveals the presence of antioxidant activity in varying degrees in many plant materials used. The high efficacy of the medicinal plants provides an alternative to conventional medicine. The cost spent on the fractionation could be avoided, if the crude extract itself has high antioxidant activity. These results may have implications in the use of the extract as a therapeutic agent in the prevention of related diseases.

Keywords

Free Radicals, Oxidative Stress, Antioxidants, Extract.

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Sonali D. Rathod ¹, P. M. Patil ¹, S. B. Jadhav ¹, P. D. Chaudhari ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Modern Collage of Pharmacy, Nigdi, Pune, IN

Abstract

Simple, sensitive, specific and economic spectrophotometric method was developed and validated for simultaneous quantitation of Metformine Hydrochloride and Pioglitazone Hydrochloride in tablet dosage form. New method based on the simultaneous estimation of drugs in a binary mixture without previous separation was developed. In simultaneous equation method, Metformine Hydrochloride and Pioglitazone Hydrochloride were quantified using their absorptivity values of at selected wavelengths, viz., 233 nm and 265.5 nm respectively. The accuracy and reproducibility of the proposed method was statistically validated by recovery studies. The simultaneous equation method permits simple, rapid and direct determination of Metformine Hydrochloride and Pioglitazone Hydrochloride in commercially available tablet dosage form without previous separations and can therefore be used for routine analysis of both drugs in quality control laboratories.

Keywords

Metformine Hydrochloride and Pioglitazone Hydrochloride, Vierodt's Method.

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Atul Phatak ¹, Pallavi Jorwekar ², P. D. Chaudhari ²

Affiliations
1 Department of Pharmaceutics, P.E.S. Modern College of Pharmacy, Nigdi, Pune-44, IN
2 P.E.S. Modern College of Pharmacy, Nigdi, Pune-44, IN

Abstract

For most of the active pharmaceutical ingredients, poor aqueous solubility and hence low bioavailability and erratic absorption is the common difficulty observed by the formulation development scientist. Nanotechnology drug delivery has gained much interest as a way to improve the solubility problems. Nanosuspension is biphasic system which consists of pure drug particles are dispersed in an aqueous vehicle of particle size less than 1000 nm, and system is stabilized by surfactant. Nanosuspension provide us with useful tool for formulating an poorly water soluble drug may be due to the properties of nanosuspension, their low production cost, ease of manufacture and scale-up. This system helps in dissolution and thus improves bioavailability of poorly water soluble drugs and holds the promise in their aspects of drug delivery. The present article reviews the various methods, manufacturing challenges and their applications as nanocarrier drug delivery system.

Keywords

Nanocarrier, Nanosuspesion, Solubility, Drug Delivery System.

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Kalyani Abhimanyu Kedar ¹, P. D. Chaudhari ², R .B. Jadhav ³, S. R. Chaudhari ⁴

Affiliations
1 Department of Pharmacognosy, Progressive Education Society's Modern College of Pharmacy, Sector -21, Yamunanagar Nigdi, Pune-411044, (MS), IN
2 Modern College of Pharmacy, Nigdi, Pune, IN
3 R.C. Patel College of Pharmacy, Shirpur, Maharastra, IN
4 Amrutvahini College of Pharmacy, Tal- Sangamner, Dist-Ahmednagar, Maharastra, IN

Abstract

The present investigation evaluates the antioxidant activity of 7-methyl-1- phenyloctan-1-one of Helicanthus elasticus Linn. Stem (Loranthaceae) in various in vitro models. The n-hexane extract of Helicanthus elasticus Linn. (Loranthaceae) parasitic on Memecylon umbellatum Burm.f. (Melastomataceae) was subjected to column chromatography. The isolated compound was characterized by UV, IR, ¹H NMR and Mass data. On comparing datas, it was confirmed that compound isolated from n-Hexane extract of Helicanthus elasticus was 7- methyl-1-phenyloctan-1-one. This is the first report stating the presence of such aromatic ketone from Helicanthus elasticus. The DPPH radical scavenging, nitric oxide scavenging and antilipid peroxidation activity of acetophenone derivative have IC₅₀ value 10.23 μg/ml, 9.73 μg/ml, 16.45 μg/ml respectively. Thus results indicate that 7- methyl-1-phenyloctan-1-one showed potent antioxidant activity compare with standards, ascorbic acid and BHA. The in vitro studies clearly indicate that the acetophenone derivative of Helicanthus elasticus Linn. Stem (Loranthaceae) has significant antioxidant activity.

Keywords

Helicanthus elasticus, Antioxidant Activity, DPPH Method, Anti-Lipid Peroxidation.

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